In this study we subjected NCX3 knockout and wildtype mice to permanent focal cerebral ischemia by intraluminal middle cerebral artery occlusion. Analysis of brain sections by 2,3,5-Triphenyl-2H-tetrazolium chloride staining, 12 hours after middle cerebral artery occlusion revealed no difference in infarct volume between NCX3 knockout and wildtype mice. In addition, we evaluated the effect of NCX3 protein knockdown on neuronal viability in primary cortical neuronal cultures following in vitro ischemia (oxygen-glucose deprivation) and L-glutamate (glutamate) exposure. In vitro experiments revealed that neuronal viability was higher in NCX3 knockout neuronal cultures than in the wildtype cultures following ischemic and glutamate insults. The reduced sensitivity of neurons from NCX3 knockout mice to in vitro ischemia and excitotoxicity indicates that NCX3 calcium entry mode activity contributes to calcium overload and neuronal death. However our in vivo finding of a lack of differential sensitivity on infarct volume in NCX3 knockout and wildtype mice suggests that any benefit of reduced NCX3 activity is overridden following permanent focal cerebral ischemia. Taken together, these findings suggest that reduced NXC3 activity limits calcium neurotoxicity during severe transient, but not during severe sustained ischemic insults. These results have important implications for the development of the NCX3 protein as a therapeutic target to reduce ischemic brain injury.

icsatc neodeme advancedstructurescorp floormachinebrush scpe jcamasonry sages-tunisie sbsteel technomailleplus bolkan vaalea nsblueprinting mycleanairdoctors nycgeneralproroofing gabgadgets prsnekkern uniquescaffoldingsystems villaguicciardini acerpackaging acjstucco prontointervento-multiservice printersupplygiant sarel takkatiimi dragonshollow mazelsupply alfalchetto reliablegeneralagency lckenterprises realizzazione-giardini dmcindustries shop chathambrass ilfaroservizi soilmechanicsdrilling colorfullyyours agcsound carriere carnavaldetournai falconauto codingxcamp davinci-trondheim thebestofcolumbia cooperativetissage outsourcedmarketingpros tlc dawnnhough doubleclick pdirealty hattrennet az-bizsolutions jamaistropdart frontdata unitycreations fortisarezzo bspo-ken codar-confection theboulders bakerpersonnel fantasyphotographyandvideo fuleky tanssikoulutria ladybi swimanddance scuolavelatoscana x-pack creativeglazing lucagnizio sigaretteelettronichepisa mayiindustries frigo-clim elgars leoemma hotelalcantara el-pro therefore thebestofjacksonville nykran masmoudi thebestofspokane cogepre detrecruitment laresidencelepartage chimneycompanyboston awarepedia res-botanica recipefy semap thebestofcincinnati bb-one fourtech thebestofjoliet ajproduce jerryshulmanproduceshipper lckcabinetryny drivingtechniquesmadeeasy accademiaestetica pizzamaison schoonheidssalonbianca cabinsbrevardnc mrbrushes atlasrolloff hatip-medikal vezcocorporation thebestofcoloradosprings filtaclean monteleeper flyfisher ellatafa associazionepugliesiapisa unitycreationsltd maketeamstudiot douglasclear packagingpro softm allstarbeerinc burtonsupply footballscouting profondacreation johnvravickddsms fireislandbuilder advancedcontrolsolutions chittorgarhtaxiservices live-now orologeriatoscana racinemode thebestofwilmington agro-services violiner sandinghouse the-complete-package thebestofrichmond rcollision meubleskarray mes-recettes lawsandtaxes tavgrupp mkproducts royscottmarine taxi contreras-stockman sigmaweb de-noord thebestofkansascity maisonmedicaledelaeken recycledrubberpavers surfacingsystems unitedfidelityinc enokplan hunterremodeling edmersupply amerequipint sanilabcorp igglesis christiannursingregistry united-royal cantodelfiume northsidedeliny menuiserie-delbart passeritartufi justmyvoice ablefiresprinklers accantoalcentro ckperformance khalfallahpneus anti-flood-barriers bmstyle autosangiorgio-mercedes-benz carmagnino viipurinurheilijat multipack cma-eng mcafeerealty adrianaperciballi loconteedilecostruzioni konarprecision unityrubberco unityrubberproducts dante tela marathongranite baltimahalworcester heimdalbygg alstateprocessservice italydreamtour wemcocastingllc yachtbritesigns ecoledecroly-renaix anchorseniorapartments royalburton sk-veilag giannacapoti hubiteg customcommercialconstruction edm-nivelles probat-tunisie bachlawyer robertex inbora gpbconstruction tekstschrijver-tim bdsit trepro himalayanlounge lazersharpplumbing nycollisionking marinanova gealcorp nygabe maritimecoverage justmyvoice jukumech quimicolsa hayat-med aldrovandiauto michaelbenaltinc korenas sandvet americanmufflerautorepair justmyvoice liacoustics dminteriors agenziapromotech ligbtour internormfirenze justmyvoice zouila kohalmiferenc ashgroveresort ilmacinapepe suzukibandit hihna islandfishli prontointerventofabbro24h thebestofmidland thebestofportland dsgnaturaeambiente rimpex-medical giadaguidi royalroseappliances normas monicavignoliniluxury reddalsand defigners projektorilamput qtbservices labandas totalconceptdesign elannonnayttamo begmaterialiedili bardsdans samuelmanndds marksmenmfg demo17 chinafinewines coralia ivar-moe tournailesbains consew luisaprofumeriashop chimneycompanywestchester danapoly 268dental uspaerospace melonerp matcoservice emperorsoft cantare fninc greenpowerchemical westendsupply domobios unitysurfacingsystems martemoen hamptonssepticservices housatonicpaper sj-transport epsl-tunisie sungoldabrasives collinscreative barbarottomachinery soep thebestofalexandria straightlineconst itcimpianti hattrem-trafikkskole federalnetworks ppattorneys ceteau fbperformance coltgateway coolservice4u garaconfection bellformalwear abcconcretepumping mysantaria accurateindustrialmachining schmugerhardware thebestofjackson bellwetherstaffing sweetkarmadesserts tonerhuset mongilschool polycliniquelaouani buonidentro schoolbusmirrorsonline qlstransportation andereuropa cleanpressiondrycleaner bsyd planetlimony thebestoflasvegas csgmfoodequip potensial potiez invitiing tomscorvetteshop thebestbaltimorebusinesses honefoss thebestofoakland hydeparkdenim scmanndds sirreal totalpreferredsupply futureshockcorp myrtun